Measurement

Part:BBa_K079026

Designed by: Maria Vittoria Amaduzzi   Group: iGEM08_Bologna   (2008-10-24)

GFP reporter protein and LacI gene sequence under the control of J23118 constitutive promoter

The GFP reporter protein was put under the control of the BBa_J23118 costitutive promoter. The reporter sequence was spaced from the promoter by the addition of the BBa_C0012 sequence without the RBS. This construct was design as an expression control plasmid for the BBa_K079020 circuit.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1188
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1864


[edit]
Categories
Parameters
None